online ISSN:2312-3389 print ISSN:2312-3370 DOI:10.15407/agrisp

Archive

Archive of Agricultural Science and Practice Journal issues

List of all issues / Content of issue 2016-1 / Abstract & References of Article 6
https://doi.org/10.15407/agrisp3.01.049
QUANTIFICATION OF TRACE ELEMENTS Fe, Zn, Mn, Se IN HULL-LESS BARLEY GRAIN
S. S. Polishchuk 1 , E. K. Kyrdohlo 1 , L. M. Mykhalska 2, B. V. Morgun 2, 3 , S. Yu. Pokhylko 3, О. І. Rybalka 2, 3, V. V. Schwartau 2

1 Institute of Selection and Genetics – National Center of Seed and Cultivar Investigation, NAAS of Ukraine
3, Ovidiopolska Road, Odesa, Ukraine, 65036

2 Plant Breeding and Genetics Institute, NAS of Ukraine
31/17, Vasylkivska Str., Kyiv, Ukraine, 03022

3 Institute of Cell Biology and Genetic Engineering, NAS of Ukraine
148, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03143

e-mail: molgen@icbge.org.ua

Received on February 15, 2016
Abstract
Aim. To explore the content of trace elements (TE), most valuable for human health – iron, zinc, manganese and selenium – in cultivars and breeding lines of hull-less barley, and to check the impact of abiotic environ- mental factors on TE accumulation in the grain of Achilles cultivar. Methods. Trace elements content was measured using ICP-MS Agilent 7700x. Results. The content of vital TE was determined in 26 samples of hull-less barley grain. The increased concentration of TE was observed in the samples with brown, blue and black caryopsis. The absence of dependence between abiotic environmental factors and TE accumulation was demonstrated using Achilles cultivar, presented by six different repeats. The correlation between the content of protein and that of iron in grain was established. Conclusions. White grain barley cultivars and lines are infe- rior in the content of Fe, Zn, Mn, and Se comparing to genotypes with brown, blue or black grain. The presence or absence of a fi lm on a hull-less barley grain has almost no impact on the amount of TE, which, as expected, are located in the aleurone layer and the germ of caryopsis. It was proven that the content of Fe, Zn, Mn in the Achilles grain almost did not change regardless of abiotic environmental factors. A high correlation, r = 0.87, Р > 0.05−0.001, was found between the content of protein and Fe in grain.
Key words: barley, microelements, iron, zinc, manganese, selenium.
References

1. Fedoroff NV. Food in a future of 10 billion. Agric & Food Secur. 2015;4:11.
https://doi.org/10.1186/s40066-015-0031-7
 
2. Breeding major food staples. Eds M. S. Kang, P. M. Priyadarshan. Oxford, Blackwell publ. 2007;437 p.
 
3. Ma JF, Higashitani A, Sato K, Takeda K. Genotypic Variation in Fe Concentration of Barley Grain Soil Sci. Plant Nutr., 2004, 50 (7):1115-7.
https://doi.org/10.1080/00380768.2004.10408583
 
4. Borrill P, Connorton JM, Balk J, Miller AJ, Sanders D, Uauy C. Biofortification of wheat grain with iron and zinc: integrating novel genomic resources and knowledge from model crops. Front Plant Sci. 2014;5:53.
https://doi.org/10.3389/fpls.2014.00053
 
5. Kaput J, Kussmann M, Mendoza Y, Le Coutre R, Cooper K, Roulin A. Enabling nutrient security and sus-tainability through systems research. Genes Nutr. 2015; 10(3):12.
https://doi.org/10.1007/s12263-015-0462-6
 
6. Hefferon KL. Nutritionally enhanced food crops; progress and perspectives. Int J Mol Sci. 2015;16(2):3895-914.
https://doi.org/10.3390/ijms16023895
 
7. Vieweger A, Döring TF. Assessing health in agriculture-towards a common research framework for soils, plants, animals, humans and ecosystems. J Sci Food Agric. 2015;95(3):438-46.
https://doi.org/10.1002/jsfa.6708
 
8. El-Haramein FJ, Grando S. Determination of iron and zinc content in food barley. Proc 10th Int Barley Genet Symp. Eds S. Ceccarelli, S. Grando (5-10 April 2008, Alexandria, Egypt). Aleppo, ICARDA. 2008;603-6.
 
9. Strain JJ, Cashman KD. Minerals and trace elements. Chapter 9. Introduction to Human Nutrition. 2nd ed. Eds M. J. Gibney, S. A. Lanham-New, A. Cassidy, H. H. Vorster. Wiley-Blackwell. 2009;188-237.
 
10. Rosado JL, Hambidge KM, Miller LV, Garcia OP, Westcott J, Gonzalez K, Conde J, Hotz C, Pfeiffer W, Ortiz-Monasterio I, Krebs NF. The quantity of zinc absorbed from wheat in adult women is enhanced by biofortification. J Nutr. 2009;139(10):1920-5.
https://doi.org/10.3945/jn.109.107755
 
11. Lyons G, Cakmak I. Agronomic biofortification of food crops with micronutrients. Fertilizing Crops to Improve Human Health: a Scientific Review. Vol. 1. Food and Nutrition Security. Paris, IFA-IPNI Publ. 2012;97-122.
 
12. Cakmak I. Enrichment of cereal grains with zinc: Agronomic or genetic biofortification? Plant and Soil. 2008;302(1):1-17.
https://doi.org/10.1007/s11104-007-9466-3
 
13. Bashir K, Takahashi R, Nakanishi H, Nishizawa NK. The road to micronutrient biofortification of rice: progress and prospects. Front Plant Sci. 2013;4:15.
https://doi.org/10.3389/fpls.2013.00015
 
14. Pilon-Smits Elizabeth AH. Selenium in Plants. Prog Bot. 2015;76:93-107.
https://doi.org/10.1007/978-3-319-08807-5_4
 
15. Persson DP, Hansen TH, Laursen KH, Husted S, Schjoerring JK. ICP-MS and LC-ICP-MS for analysis of trace element content and speciation in cereal grains. Methods Mol Biol. 2012;860:193-211.
https://doi.org/10.1007/978-1-61779-594-7_13